p53 expression confers sensitivity to 5‑fluorouracil via distinct chromatin accessibility dynamics in human colorectal cancer
نویسندگان
چکیده
One of the most commonly used drugs in chemotherapy, 5‑fluorouracil (5‑FU) has been shown to be effective only 10‑15% patients with colon cancer. Thus, studies mechanisms affecting 5‑FU sensitivity these are necessary. The tumor suppressor protein p53 is a transcription factor that serves important roles cell apoptosis by regulating cycle. It also characterized as key influencing drug sensitivity. Furthermore, accessible chromatin hallmark active DNA regulatory elements and functions crucial epigenetic cancer mechanisms. present study assessed genetic landscape performing RNA sequencing Assay for Transposase‑Accessible Chromatin sequencing, investigated effects on accessibility gene expression. Notably, while treatment mediated global increases accessibility, organization several genomic regions differed depending expression status p53. Since occupancy does not overlap regions, 5‑FU‑mediated changes were regulated direct binding In p53‑expressing condition, region was primarily associated genes encoding death pathways. Additionally, revealed induce open conformation at containing motifs AP‑1 family factors, which may drive pathway genes. conclusion, confer distinct transcription‑independent manner.
منابع مشابه
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ژورنال
عنوان ژورنال: Oncology Letters
سال: 2021
ISSN: ['1792-1074', '1792-1082']
DOI: https://doi.org/10.3892/ol.2021.12487